ABSTRACT
Uncinula necator and Botrytis cinerea are the most destructive pathogens of the grapevine in Tunisia and elsewhere. We used two strains of Bacillus subtilis group, B27 and B29 to control powdery mildew and the grey mold disease of the grapevine. Green house experiments showed that B29 and B27 strains of the bacteria efficiently reduced the severity of powdery mildew up to 50% and 60%, respectively. Further, they decreased Botrytis cinerea development on grape leaf by 77% and 99%, respectively. The mode of action has been shown to be chitinolytic. These two bacteria showed significant production of total proteins discharged into the culture medium. Determination of some chitinolytic enzymes revealed the involvement of N-acetyl glucosaminidase (Nagase), the chitin-1,4-chitobiosidase (Biase) and endochitinase in degrading the mycelium of B. cinerea.
Subject(s)
Acetylglucosaminidase/metabolism , Antibiosis/physiology , Ascomycota/chemistry , Ascomycota/physiology , Bacillus subtilis/classification , Bacillus subtilis/enzymology , Bacillus subtilis/physiology , Bacterial Proteins/metabolism , Botrytis/chemistry , Botrytis/physiology , Chitin/metabolism , Chitinases/metabolism , Culture Media, Conditioned/metabolism , Hexosaminidases/metabolism , Host-Pathogen Interactions , Plant Diseases/microbiology , Species Specificity , Time Factors , Vitis/microbiologyABSTRACT
Les registres des cancers constituent de plus en plus une source majeure d'information sur le profil de cette maladie dans une localite donnee. Ici a ete appliquee la technique; qui est d'ailleurs clairement expliquee; de recolte des informations pour editer le registre des cancers de Serif en Algerie. Les auteurs ont principalement insiste sur l'incidence et la survie. Chez l'homme le taux le plus eleve concerne les cancers bronchopulmonaires (18;3) et chez les femmes les cancers du sein avec 18;8